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C57BL/6 mice were immunized with EL4 thymoma cells and the spleen cells of the immunized animals were fused with the cells of mice bearing the hemolytic variant EL4/23 thymoma. The cells of the resulting hybridomas were then grown in media containing the macrophage-activating agent Corynebacterium parvum. The hybridomas produced antibodies that were of the IgM class and were reactive with EL4 cells. The antibodies were opsonized by the Fc portion of their immune complexes and they lysed EL4 cells in a complement-dependent manner (CDC) by a mechanism that involved both anti-Fc receptor and complement on the surface of the target cells. The antibody-dependent cellular cytotoxicity (ADCC) by the lymphocyte-derived effector cells used in the fusion was enhanced when the target cells were labeled with the I125-radiolabeled antibody, as compared with the unlabeled cells. The enhancement was only seen when the target cells were sensitized to the anti-EL4 antibody. The data suggested that the antibodies adsorbed on the EL4 cells might enhance the antibody-dependent cellular cytotoxicity by increasing the binding of lymphocytes to the target cells. The results also suggested that opsonized tumor cells could be taken up by APC by endocytosis and that the resultant antigen-specific T cell stimulation might increase the ADCC of macrophages by the T cell-derived effector cell./* Copyright (c)
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* This software is provided ‘as-is’, without any express or implied
* warranty. In no event will the authors be held liable for any damages
* arising from the use of this software.
*
* Permission is granted to anyone to use this software for any purpose,
* including commercial applications, and to alter it and redistribute it
*

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